Research Technician I

The University Health Network, where "above all else the needs of patients come first", encompasses Toronto General Hospital, Toronto Western Hospital, Princess Margaret Cancer Centre, Toronto Rehabilitation Institute and the Michener Institute of Education. The breadth of research, the complexity of the cases treated, and the magnitude of its educational enterprise has made UHN a national and international resource for patient care, research and education. With a long tradition of ground breaking firsts and a purpose of "Transforming lives and communities through excellence in care, discovery and learning", the University Health Network (UHN), Canada's largest research teaching hospital, brings together over 16,000 employees, more than 1,200 physicians, 8,000+ students, and many volunteers. UHN is a caring, creative place where amazing people are amazing the world.

Union: Non-Union
Site: Princess Margaret Cancer Centre
Department: Division of Medical Oncology and Hematology
Reports to: Clinician Scientist
Work Model: On-Site
Grade:V0:90
Hours: 37.5 hours per week
Salary: $25.98 - $32.48 per hour: To commensurate with experience and consistent with UHN compensation policy
Shift: Monday-Friday
Status: Temporary Full-Time (1 year- renewable)
Closing Date: November 15, 2024

Position Summary:

A Research Technician position is available in Toronto, Canada, in the laboratory of Dr. Tomohiro Aoki at the Princess Margaret Cancer Centre, University Health Network; one of the top five cancer research centres in the world. The research focus is basic/translational research in cancer immunology and cancer genomic field in the lymphoid malignancy. The successful applicant will utilize standardized laboratory techniques and procedures in immunology, genomics and molecular biology applications; perform experiments and analysis; communicate and maintain records of experimental data.

Duties:

• Will utilize standardized laboratory techniques and procedures in immunology, genomics and molecular biology applications including DNA/RNA/Protein extraction, library preparation, immune blotting and flow cytometry.
• Will perform experiments and analysis; communicate and maintain records of experimental data.
• Manage and oversee the mouse colony, including breeding, weaning, and maintaining health records.
• Perform genotyping of mice to identify genetic variations and ensure proper lineage.
• Assist in experimental procedures such as mouse dissection, blood collection, and tissue processing.
• Support lab organization by maintaining equipment and supplies and ensuring a clean and safe working environment.
• Handle inventory management tasks, including stocking necessary reagents and materials.
• Work onsite five days a week, with the flexibility to adapt to varying schedules as needed.

• At minimum, BSc in molecular biology, immunology, or related discipline
• At minimum, 3 years related experience.
• Demonstrated excellent organizational and time management skills are required.
• Experience with mice handling and/or colony maintenance (breeding, weaning, ear tagging, injections, bleeding, etc.) is required
• Experience in basic cancer immunology assays including Handling of single channel and multichannel pipettes, manual counting of cells and nuclei via cytometer. flow cytometry, cell cultures, virus transfection/transduction, DNA/RNA/ extraction, immune blotting, PCR and CRISPR\
• Experience in vivo assay using mouse including in vivo imaging, iv injection, mouse dissection, blood collection, and tissue processing.
• Experience in protein purification, characterization, and functional assays
• Proficiency in use of microscopy to study imaging analyses is a plus.
• Familiarity with biochemical and biophysical methods
• Experience with mouse genotyping or willingness to learn
• Experience with mouse dissection, organ isolation and processing
• Strong background with Unix/Linux, Perl and/or Python is a plus.
• Familiarity with genomic/spatial data including single cell RNA sequencing, imaging analysis and NGS platforms and datatypes (FASTQ, BAM, VCF, etc) is a plus.
• Experience with analysis of targeted, exome or genome sequencing data is a plus
• A willingness to learn new techniques and technologies is required.
• Meticulous record-keeping and attention to detail
• Excellent organizational and communication skills
• Ability to work individually as well as part of a team

Why join UHN?

In addition to working alongside some of the most talented and inspiring healthcare professionals in the world, UHN offers a wide range of benefits, programs and perks. It is the comprehensiveness of these offerings that makes it a differentiating factor, allowing you to find value where it matters most to you, now and throughout your career at UHN.

• Competitive offer packages
• Government organization and a member of the Healthcare of Ontario Pension Plan (HOOPP https://hoopp.com/)
• Close access to Transit and UHN shuttle service
• A flexible work environment
• Opportunities for development and promotions within a large organization
• Additional perks (multiple corporate discounts including: travel, restaurants, parking, phone plans, auto insurance discounts, on-site gyms, etc.)

Current UHN employees must have successfully completed their probationary period, have a good employee record along with satisfactory attendance in accordance with UHN's attendance management program, to be eligible for consideration.

All applications must be submitted before the posting close date.

UHN uses email to communicate with selected candidates. Please ensure you check your email regularly.

Please be advised that a Criminal Record Check may be required of the successful candidate. Should it be determined that any information provided by a candidate be misleading, inaccurate or incorrect, UHN reserves the right to discontinue with the consideration of their application.

UHN is an equal opportunity employer committed to an inclusive recruitment process and workplace. Requests for accommodation can be made at any stage of the recruitment process. Applicants need to make their requirements known.

We thank all applicants for their interest, however, only those selected for further consideration will be contacted.