Research Technician II

The University Health Network, where "above all else the needs of patients come first", encompasses Toronto General Hospital, Toronto Western Hospital, Princess Margaret Cancer Centre, Toronto Rehabilitation Institute and the Michener Institute of Education. The breadth of research, the complexity of the cases treated, and the magnitude of its educational enterprise has made UHN a national and international resource for patient care, research and education. With a long tradition of ground breaking firsts and a purpose of "Transforming lives and communities through excellence in care, discovery and learning", the University Health Network (UHN), Canada's largest research teaching hospital, brings together over 16,000 employees, more than 1,200 physicians, 8,000+ students, and many volunteers. UHN is a caring, creative place where amazing people are amazing the world.

Union: Non-Union
Site: Princess Margaret Cancer Centre
Department: Princess Margaret Genomics Centre
Reports to: Manager, PM Genomics Centre
Work Model: On-Site
Grade: B0:05
Hours: 37.5 hours per week
Salary: $57,018 - $71,273 annually: To commensurate with experience and consistent with UHN compensation policy
Shifts: Monday - Friday
Status: Temporary Full Time (18 months contract)
Closing Date: October 31, 2024

Position Summary
The Princess Margaret Genomics Centre is seeking a temporary full-time (maternity leave cover) Research Technician II. Working under the direction of senior staff, the successful applicant will utilize standardized laboratory techniques and procedures in genomics and molecular biology applications; perform experiments and analysis; communicate and maintain records of experimental data. As an integral team member at the Genomics Centre, a UHN core facility, the research technician must be dedicated and result-driven and will be involved in all aspects of service projects, following SOPs and meeting target deadlines. There will be a special emphasis on techniques for single nucleus RNA-seq and single nucleus ATAC-seq.

Duties

Working under the direction of senior staff, the successful applicant:

• Will utilize standardized laboratory techniques and procedures in genomics and molecular biology applications.
• Will perform experiments and analysis; communicate and maintain records of experimental data.
• Will be a key member of a team focused on single nucleus genomics utilizing Multiome, ATAC, and 3' and 5' profiling assays from 10x Genomics. Assays require isolation and purification of nuclei from solid tissue or cell suspensions followed by specific assay workflows.

• At minimum, BSc in genomics, molecular biology or related discipline is required
• At least 3 years of experience with standard molecular biology laboratory techniques is required, such as:
  • PCR reaction set up and purification.
  • Nucleic acid quantification by spectrophotometry and fluorimetry.
  • Handling of single channel and multichannel pipettes.
  • Manual counting of cells and nuclei via cytometer.
• A willingness to learn new techniques and technologies is required.
• Demonstrated excellent organizational and time management skills are required.
• Ability to work independently and accurately under deadlines is required.
• Meticulous and detail-oriented lab performance is required.
• Experience with 10x Genomics assays including Multiome, ATAC, 3' and 5' is an asset.
• Experience with nuclei extraction from fresh or frozen solid tissue and cells is an asset.
• Excellent interpersonal and communication skills in a team environment is required.
• Experience with using LIMS (Laboratory Information Management System) is an asset.
• Experience with Illumina DNA sequencing library construction workflows is an asset.

Why join UHN?

In addition to working alongside some of the most talented and inspiring healthcare professionals in the world, UHN offers a wide range of benefits, programs and perks. It is the comprehensiveness of these offerings that makes it a differentiating factor, allowing you to find value where it matters most to you, now and throughout your career at UHN.

• Competitive offer packages
• Government organization and a member of the Healthcare of Ontario Pension Plan (HOOPP https://hoopp.com/)
• Close access to Transit and UHN shuttle service
• A flexible work environment
• Opportunities for development and promotions within a large organization
• Additional perks (multiple corporate discounts including: travel, restaurants, parking, phone plans, auto insurance discounts, on-site gyms, etc.)

Current UHN employees must have successfully completed their probationary period, have a good employee record along with satisfactory attendance in accordance with UHN's attendance management program, to be eligible for consideration.

All applications must be submitted before the posting close date.

UHN uses email to communicate with selected candidates. Please ensure you check your email regularly.

Please be advised that a Criminal Record Check may be required of the successful candidate. Should it be determined that any information provided by a candidate be misleading, inaccurate or incorrect, UHN reserves the right to discontinue with the consideration of their application.

UHN is an equal opportunity employer committed to an inclusive recruitment process and workplace. Requests for accommodation can be made at any stage of the recruitment process. Applicants need to make their requirements known.

We thank all applicants for their interest, however, only those selected for further consideration will be contacted.